Inkcazo
β-Amyloid (1-28) yi aβ-iqhekeza le-amyloid elinokuthi lithathe inxaxheba ekubopheni isinyithi.I-Beta-amyloid yi-peptide eyenza i-amyloid plaques kwingqondo yezigulane ezinesifo se-Alzheimer's.1. Nyibilikisa i-A eqinileyoβ i-peptide kwi-hexafluoro-2-propanol ebandayo (HFIP).I-Peptides yafakwa kwiqondo lokushisa kwegumbi ubuncinane kwi-1 h ukuseka i-monomerization kunye ne-randomization yezakhiwo.2. I-HFIP yasuswa ngumphunga kwaye i-peptide efunyenweyo yagcinwa njengefilimu kwi -20 okanye -80° C. 3 Iimbrane ezibangelwayo zachithwa kwi-5mM anhydrous DMSO kwaye emva koko i-vortexed kwaye ihlanjululwe kwi-concentration efanelekileyo kunye ne-buffer (ephakathi ngaphandle kwe-serum kunye ne-phenol ebomvu).4 Emva koko, isisombululo sasineminyaka emi-4-8° C iiyure ezingama-48.Iisampulu emva koko zafakwa kwi-centrifuged kwi-14000g ye-10 min kwi-4-8.° C. Iioligomers ezinyibilikayo zazikwiqondo eliphezulu.I-supernatant yaxutywa ka-10 ukuya kwi-200-fold for experiments.
Iinkcukacha
Imbonakalo: Imhlophe ukuya kumgubo omhlophe
Ubunyulu(HPLC):≥98.0%
Ukungacoceki okukodwa:≤2.0%
Umxholo we-Acetate(HPLC): 5.0%~12.0%
Isiqulatho samanzi (uKarl Fischer):≤10.0%
Umxholo wePeptide:≥80.0%
Ukupakisha kunye nokuThumela: Ukushisa okuphantsi, ukupakisha i-vacuum, ngokuchanekileyo kwi-mg njengoko kufuneka.
Ungaodola Njani?
1. Contact us directly by phone or email: +86-13735575465, sales1@gotopbio.com.
2. Oda kwi-intanethi.Nceda ugcwalise ifom ye-odolo ye-intanethi.
3. Nika igama le-peptide, inombolo ye-CAS okanye ulandelelwano, ukucoceka kunye nokuguqulwa ukuba kuyimfuneko, ubungakanani, njl.njl. siya kubonelela ngekowuteshini kwiiyure ezi-2.
4. Umyalelo wokuthotyelwa kwemvumelwano yokuthengisa esayiniweyo ngokufanelekileyo kunye ne-NDA (isivumelwano esingaveziyo) okanye isivumelwano esiyimfihlo.
5. Siya kuqhubeka sihlaziya inkqubo ye-odolo ngexesha.
6. Ukuhanjiswa kwePeptide yi-DHL, Fedex okanye abanye, kunye ne-HPLC, MS, COA iya kubonelelwa kunye nomthwalo.
7. Umgaqo-nkqubo wembuyekezo uya kulandelwa ukuba kukho ukungangqinelani komgangatho okanye inkonzo yethu.
8. Inkonzo emva kokuthengisa: Ukuba abathengi bethu banemibuzo malunga nepeptide yethu ngexesha lovavanyo, nceda uzive ukhululekile ukuqhagamshelana nathi kwaye siya kuyiphendula ngexesha elifutshane.
Zonke iimveliso zenkampani zisetyenziselwa kuphela injongo yophando lwesayensi, yona'Ithintelwe ukuba isetyenziswe ngokuthe ngqo nguye nawuphi na umntu emzimbeni womntu.
FAQ:
Zeziphi iingcebiso ukuba ndiqala ukusebenzisa iipeptides?
Xa ulungele ukusetyenziswa, landela la manyathelo angezantsi ukunyibilikisa iipeptides ukugcina umgangatho wazo.
I-1, ngaphambi kokuvula ibhotile kwaye ulinganise inxalenye yepeptide, yifudumeze ukufikelela kwiqondo lokushisa, kwaye ixesha lokufudumeza liyacetyiswa ukuba libe yiyure enye.
2. Ngokukhawuleza ulinganise inani elifunekayo kwindawo ecocekileyo yangaphandle.
3. Gcina iipeptides eziseleyo kwisikhenkcisi esingaphantsi kwama-20℃, yongeza i-desiccants kwaye uyigcine kwisitya esingenawo umoya.
Ndihlala phesheya, kwaye kuya kuthatha iintsuku ezininzi ukuhanjiswa kunye nokukhutshwa kwamasiko.Ngaba oku kuya kuluchaphazela uphando lwam?
Ufumana iipeptides kwiipakethi zomgubo we-lyophilized, kwaye iipeptide zinokugcinwa kwiqondo lobushushu begumbi ngaphandle komonakalo.Nceda yiba ngumkhenkce kwaye uyigcine kwangoko emva kokuba uyifumene.
Ziziphi iingxaki ekufuneka ziqwalaselwe kwinkqubo yokugcina?
Ipeptide oyifumeneyo ibipakishwe kumgubo ocoliweyo.Iipeptides ziyi-hydrophilic, kwaye ukufunxwa kwamanzi kuya kunciphisa ukuzinza kwe-peptide kunye nokunciphisa umxholo wepeptide.Nceda uqaphele oku kulandelayo: okokuqala, kunye nama-desiccants, agcinwe kwindawo eyomileyo.Okwesibini, xa ifunyenwe, nceda ngokukhawuleza uyifake kwisikhenkcisi -20℃yokugcina, ukuze kugcinwe uzinzo oluphezulu.Okwesithathu, kuphephe ukusebenzisa iqabaka ngokuzenzekelayo kwifriji.Ukutshintsha kokufuma kunye nobushushu kunokuchaphazela ukuzinza kweepeptides.Okwesine, ukushisa kwangaphandle ngexesha lokuthutha akuchaphazeli ukuqinisekiswa kunye nomgangatho we-peptides.
Ndizigcina njani iipeptide ezikhenkcezisiweyo xa ndifumana imveliso?
Nje ukuba uyifumene, kufuneka uyigcine kwangoko ku -20° C okanye ngaphantsi.
Ukuba umxholo we-peptide yi-80%, yintoni enye i-20%?
Ityuwa kunye namanzi
Ukuba i-peptide icocekile ngama-98%, yintoni i-2%?
Iipesenti ezimbini zokwakheka zacuthwa okanye zacinywa amaqhekeza olandelelwano.
Yintoni iyunithi ye-AMU?
I-AMU yiyunithi ye-micropolymerization.Le yiyunithi jikelele yomlinganiselo weepeptides.