Isikimu soyilo kunye nesisombululo sekhonkco le-polypeptide peptide

I. Isishwankathelo
Iipeptides ziimacromolecules ezikhethekileyo kangangokuba ulandelelwano lwazo aluqhelekanga kwiikhemikhali zazo kunye neempawu zomzimba.Ezinye iipeptides kunzima ukuzidibanisa, ngelixa ezinye kulula ukuzidibanisa kodwa kunzima ukuzicoca.Ingxaki esebenzayo yeyokuba uninzi lweepeptides lunyibilika kancinane kwizisombululo ezinamanzi, ke ngoko ekusulungekisweni kwethu, indawo ehambelanayo ye-hydrophobic peptide kufuneka inyibilikiswe kwizinyibilikisi ezingenamanzi, ke ngoko, ezi zinyibilikisi okanye izithinteli zinokungahambelani kakhulu nokusetyenziswa. kweenkqubo zovavanyo lwebhayoloji, ukuze amagcisa athintelwe ngokungqongqo ekusebenziseni i-peptide ngeenjongo zabo, ukuze oku kulandelayo yimiba emininzi yoyilo lweepeptides kubaphandi.

Isikimu soyilo kunye nesisombululo sekhonkco le-polypeptide peptide
Okwesibini, ukhetho oluchanekileyo lweepeptides ezinzima zokwenziwa
1. Ubude obupheleleyo bolandelelwano oluphantsi kolawulo
Iipeptide ezingaphantsi kwe-15 iintsalela kulula ukuzifumana kuba ubukhulu bepeptide buyanda kwaye ubunyulu bemveliso ekrwada buyancipha.Njengoko ubude bubonke betsheyini lepeptide bunyuka ngaphaya kweentsalela ezingama-20, ubungakanani bemveliso obuchanekileyo yeyona nto ixhalabisayo.Kwimifuniselo emininzi, kulula ukufumana iziphumo ezingalindelekanga ngokuthoba inani lentsalela ngaphantsi kwama-20.
2. Ukunciphisa inani leentsalela ze-hydrophobic
I-Peptides ene-predominance enkulu yeentsalela ze-hydrophobic, ngokukodwa kwingingqi ye-7-12 i-residus esuka kwi-C-terminus, ngokuqhelekileyo ibangela ubunzima bokwenziwa.Oku kubonwa njengendibaniselwano enganelanga ngokuchanekileyo ngenxa yokuba ishidi le-B-fold lifunyenwe kwi-synthesis.Kwiimeko ezinjalo, kunokuba luncedo ukuguqula ngaphezulu kweentsalela ezimbini ezilungileyo nezingalunganga, okanye ukubeka iGly okanye iPro kwipeptide ukuvula ukwakheka kwepeptide.
3. Ukulawulwa kweentsalela "ezinzima".
"Kukho inani leCys, Met, Arg, kunye neZama iintsalela ezingadityaniswanga ngokulula."I-Ser iya kusetyenziswa njengenye indlela engaxoxisiyo kwiiCys.
Isikimu soyilo kunye nesisombululo sekhonkco le-polypeptide peptide

Okwesithathu, phucula ukhetho oluchanekileyo lokunyibilika emanzini
1. Lungisa i-N okanye i-C terminus
Ngokubhekiselele kwiipeptide ezine-acidic (oko kukuthi, ukuhlawuliswa kakubi kwi-pH 7), i-acetylation (i-N-terminus acetylation, i-C terminus isoloko igcina iqela lamahhala le-carboxyl) iyacetyiswa ngokukodwa ukunyusa intlawulo embi.Nangona kunjalo, kwi-peptides esisiseko (oko kukuthi, ihlawuliswe ngokufanelekileyo kwi-pH 7), i-amination (iqela le-amino lamahhala kwi-N-terminus kunye ne-amination kwi-C-terminus) iyacetyiswa ngokukodwa ukunyusa intlawulo efanelekileyo.

2. Nciphisa kakhulu okanye andise ulandelelwano

Ezinye zeendlela ezilandelelanayo ziqulethe inani elikhulu le-hydrophobic amino acids, njengeTrp, Phe, Val, Ile, Leu, Met, Tyr kunye ne-Ala, njl. Xa ezi ntsalela ze-hydrophobic zidlula i-50%, ngokuqhelekileyo akulula ukuzinyibilikisa.Kunokuba luncedo ukwandisa ulandelelwano ukwandisa ngakumbi iipali ezilungileyo nezingalunganga zepeptide.Inketho yesibini kukunciphisa ubungakanani bekhonkco le-peptide ukwandisa iipali ezilungileyo kunye nezibi ngokunciphisa iintsalela ze-hydrophobic.Okukhona omelele amacala alungileyo nangalunganga wekhonkco le peptide, kokukhona kunokwenzeka ukuba asabele ngamanzi.
3. Beka kwintsalela enyibilikayo emanzini
Kwamanye amatsheyini e-peptide, indibaniselwano yee-amino acids ezilungileyo nezingalunganga zinokuphucula ukunyibilika kwamanzi.Inkampani yethu icebisa i-N-terminus okanye i-C-terminus ye-peptides acidic ukuba idityaniswe neGlu-Glu.I-N okanye i-C terminus ye-peptide esisiseko yanikwa kwaye emva koko iLys-Lys.Ukuba iqela elihlawulisiweyo alikwazi ukubekwa, i-Ser-Gly-Ser nayo inokubekwa kwi-N okanye i-C terminus.Nangona kunjalo, le ndlela ayisebenzi xa amacala ekhonkco yepeptide engenakuguqulwa.