Ngokwendlela yokudibanisa ye-amino acid kunye noshukela, i-peptide yeswekile inokwahlulwa ibe ngamacandelo amane: i-O glycosylation, i-C a N glycosylation, i-saccharification yamazolo kunye ne-GPI (glycophosphatidlyinositol) uxhumano.
1. I-N-glycosylation glycopeptides yenziwe nge-N-acetamide i-glucose ekupheleni kokunciphisa i-glycan chain (i-Glc-Nac) edityaniswe ne-athomu ye-N kwiqela le-amide yecala lecala le-Asn kwi-peptide chain, kunye ne-Asn. ekwazi ukudibanisa ikhonkco ye-glycan kufuneka ibekwe kwi-AsN-X-Ser / Thr (X! = P) kwi-motif eyenziwe ngama-residu.Iswekile yiN-acetylglucosamine.
I-N-glycosylation eguqulelwe kwi-structural glycopeptide
2. Isakhiwo se-O-glycosylation silula kune-N-glycosylation.Le glycopeptide imfutshane ngokubanzi kune-glycan, kodwa ineentlobo ezininzi kune-N-glycosylation.I-Ser kunye ne-Thr ngokubanzi zinokufakwa kwi-glycosylated kwikhonkco le-peptide.Ukongeza, kukho i-glycopeptides ehonjiswe nge-tyrosine, i-hydroxyl, kunye ne-hydroxyproline glycosylation.Indawo yekhonkco yi-athomu ye-oksijini ye-hydroxyl kwicala lekhonkco lentsalela.Iishukela ezidibeneyo ziyi-galactose okanye i-N-acetylgalactosamine (i-Gal & GalNAc) okanye i-glucose / glucosamine (Glc / GlcNAc), i-mannose / mannosamine (Man / ManNAc), njl.
I-O-glycosylation iguqula isakhiwo
3. I-Glycopeptide O-GlcNAC glycosylation ((N-acetylcysteine (NAC)) (glcnAcN-acetylglucosamine/acetylglucosamine)
I-N-acetylglucosamine enye (GlcNAc) i-glycosylation idibanisa iiprotheni ze-O-GlcNAc kwi-athomu ye-oksijini ye-hydroxyl ye-serine okanye i-threonine intsalela yeprotheni.I-O-GlcNA glycosylation yi-O-GlcNAc monosaccharide ihombiso ngaphandle kokwandiswa kwe-glycan;Njenge-peptide phosphorylation, i-O-GlcNAc glycosylation ye-glycopeptides ikwayinkqubo yokuhombisa iprotein eguqukayo.Ukuhonjiswa okungaqhelekanga kwe-O-GlcNAc kunokubangela izifo ezahlukeneyo ezifana nesifo seswekile, izifo zentliziyo, ithumba, isifo sika-Alzheimer njalo njalo.
Amanqaku e-glycosylation ye-glycopeptides
Izakhiwo ezisisiseko ze-polypeptide kunye ne-sugar chain zidibaniswe namatyathanga eprotheyini ngamabhondi adibeneyo, kwaye iziza ezidibanisa amatyathanga eswekile zibizwa ngokuba ziindawo ze-glycosylation.Ekubeni akukho template yokulandela i-biosynthesis ye-glycopeptide sugar chain, i-sugar chain ezahlukeneyo ziya kuqhotyoshelwa kwindawo efanayo ye-glycosylation, ekhokelela kwinto ebizwa ngokuba yi-microscopic inhomogeneity.
I-glycosylation ye-glycopeptides
1. Impembelelo ye-glycopeptide glycosylation kunyango-impumelelo yeeproteni zonyango.
Kwimeko yeeprotheyini zonyango-zonyango, i-glycosylation ikwachaphazela isiqingatha sobomi kunye nokujoliswa kweziyobisi zeprotheyini kwi-vivo.
2. I-glycopeptide e-Soluble glycosylation kunye neeprotheni
Uphononongo lubonise ukuba amatyathanga eswekile kumphezulu weeproteni anokuphucula ukunyibilika kwemolekyuli yeeproteni
3. I-Glycopeptide glycosylation kunye ne-protein immunogenicity
Kwelinye icala, amatyathanga eswekile kumphezulu weeproteni anokubangela iimpendulo ezithile zokuzikhusela kwizifo.Kwelinye icala, amatyathanga eswekile anokugquma iindawo ezithile kumphezulu weprotein kwaye anciphise ukungazinzi kwawo
4. I-Glycopeptide glycosylation eyandisa ukuzinza kweprotheni
I-Glycosylation inokunyusa ukuzinza kweeprotheni kwiimeko ezahlukeneyo ze-denaturation (ezifana ne-denaturants, ukushisa, njl.) kwaye ugweme ukuhlanganiswa kweeprotheni.Kwangaxeshanye, imixokelelwane yeswekile kumphezulu weeproteni inokugquma iindawo zokuwohloka kweproteolytic yeemolekyuli zeprotheyini, ngaloo ndlela inyusa ukuxhathisa kweeprotheyini kwiiprotheyini.
5. I-Glycopeptide glycosylation echaphazela umsebenzi webhayoloji weemolekyuli zeprotheni
Ukutshintsha iprotein glycosylation kunokwenza ukuba iimolekyuli zeprotheyini zenze imisebenzi emitsha yebhayoloji
Ixesha lokuposa: Aug-03-2023