Ukuguqulwa kwe-methylation

I-peptides eguqulwe i-Methylation, eyaziwa ngokuba yi-methylation-recognized peptides, ziyiprotheyini yokuhlobisa emva kokuguqulela (PTMs) kwaye idlala indima ebalulekileyo yokulawula phantse yonke imisebenzi yobomi kwiiseli.Iiprotheyini zicutshungulwa yi-methyltransferase ukuhambisa amaqela e-hydroxyl kwiintsalela ze-amino acid ezithile ukuze zibopheleleke.I-Methylation yinkqubo yokuguqula ukuguqulwa eyenziwa yi-demethylases.

Uphononongo lubonise ukuba i-methylated / demethylated amino acids eqhelekileyo idla ngokuba yi-lysine (Lys) kunye ne-arginine (Arg).Izifundo ziye zafumanisa ukuba i-histone lysine methylation inemisebenzi eyahlukeneyo yezinto eziphilayo ezifana nokugcinwa kwe-stem cell kunye nokwahlula, ukungasebenzi kwe-chromosome ye-X, ukulawulwa kwe-transcription kunye nempendulo yomonakalo we-DNA.", ihlala ichaphazela i-chromatin condensation kwaye icinezela ukubonakaliswa kofuzo."I-Histone arginine methylation idlala indima ebalulekileyo ekulawuleni i-gene transcription kwaye inokuchaphazela iinkqubo ezahlukeneyo ze-physiological kwiiseli, kubandakanywa ukulungiswa kwe-DNA, ukuhanjiswa komqondiso, ukuphuhliswa kweeseli kunye ne-carcinogenesis.Ke ngoko, i-Guopeptide Biology iye yaphuhlisa ngokukodwa itekhnoloji ye-methyl yokuhombisa i-peptides, eguqulwa ngoososayensi emva kokuguqulelwa kweprotheyini (PTMS) ukunceda kuphando.

Ukuguqulwa kweMethylation (Me1, Me2, Me3)

Umgangatho ophezulu we-Fmoc-Ls(Me, Boc)-OH, Fmoc-Lys(Me2)-OH, Fmoc-Lys(Me3)-OH.HCL, Fmoc-Arg(Me,Pbf)-OH, Fmoc-Arg(Me) I-2-OH.HCl (i-asymmetrical), i-F yayisetyenziselwa i-moc-Arg (me) i-2-OH.HCl (i-symmetrical) kunye nezinye izinto eziluhlaza zenziwe yinkqubo ye-FMOC yesigaba esiqinileyo sokudibanisa ukufumana i-Lys kunye ne-Arg methylated peptides, kunye neemveliso. zahlanjululwa yi-HPLC.I-spectra yobunzima obufanelekileyo, iichromatogram ze-HPLC kunye ne-COA zinikezelwe kwimveliso egqityiweyo.