Indlela entsha ye-Thymus yenziwe nge-28 amino acid synthetic polypeptide immune regulator.Indlela ye-Thymus luhlobo lwamachiza amatsha e-uct, ahlala esetyenziswa kwizigulana zethumba, anokonyusa ukhuselo lomzimba.
Indlela yokwenza
Indlela echanekileyo ye-thymalfasin kunyango lwe-hepatitis B engapheliyo ayizange ifundwe ngokupheleleyo, kodwa iimvavanyo ezahlukeneyo zibonise ukuba i-thymalfasin inempendulo ebalulekileyo ye-immune.Ifezekiswa ikakhulu ngokuphucula ukusebenza kweeseli T, kwaye ihamba ngeemeko ezibandakanya ukuveliswa kweeseli ze-T, ulwahlulo kunye nokuphuculwa, kunye ne-apoptosis.Ngokwandisa inani elipheleleyo le-lymphoid, umbulali wendalo we-intrahepatic, i-CD3 kunye neeseli ze-CD4, i-thymalfasin inokudlala indima ye-immunomodulatory.I-Pleural fasin inokukhuthaza ukukhula kunye nokwahlulwa kweeseli ze-DC, kwaye ngaloo ndlela ikhuthaze ukukhula kunye nokwahlulahlula kweeseli ze-T.I-Thymalfasin ingenza i-mitogen okanye i-antigen isebenze, iphucule ukuveliswa kwee-cytokines ezahlukeneyo ezifana ne-interferon human interleukin IL2 kunye ne-IL3, iphucula ukubonakaliswa kwe-interleukin-2 receptor, kwaye yenze iiseli ze-CD4T zahluke kwi-THLS, ezineziphumo ezibalulekileyo ze-antiviral.Ngokwandisa indima yeeseli ze-P, inokukhuthaza ukuveliswa kwe-lymphocytes kwi-vivo, ilungelelanise umlinganiselo we-P cell subsets kunye nokwahlukana kweeseli ze-U, kunye nokuveliswa kwe-antibodies, ukukhuthaza ukuveliswa kwamanye ama-lymphokines kunye ne-macrophages, ukuphucula ukugwinya. umsebenzi, kunye nokuphucula umsebenzi we-immune system kwizigulane ezinomhlaza.Iyakwazi ukususa iibhaktheriya, ibhaktheriya kunye neentsholongwane emzimbeni.Incwadi yendlela ye-Thymus yenkqubo ye-nervous kunye ne-endothelium ejikelezileyo, ukuphakama ukulungelelanisa indima ye-hippocampal neuron ye-synaptic transmission.Indlela ye-Thymus entsha yosasazo lwe-synaptic kunye ne-nervous system, ingasetyenziselwa ukuthintela izigulana zomhlaza ezibangelwa lunyango lomhlaza olufana nechemotherapy kunye ne-neurotoxicity.I-Pleuraxin ineempembelelo zokulawula imfuza, inokubangela i-kinase cascade genes, ikhulise i-cytokine gene, i-immune response regulatory genes.Ulawulo oluphezulu lwe-thymalfasin kwi-IL-like receptor genes icebisa ukuba i-thymalfasin ivuselela iiseli ezibonisa i-antigen."Ngaphezu koko, impembelelo ye-thymalfasin ekukhuleni kweeseli ze-immune ibonakaliswe ekuboniseni ukwanda kweeseli ze-stem kunye ne-hematopoietic protein gene."
Ulandelelwano olutsha lwe-thymectomy
Inkqubo yokulungiselela
1. Indlela ye-Biochemical extraction Amalungiselelo e-Thymosin (umxube we-peptides) afunyanwa ngokusisiseko kwizicubu zezilwanyana, ngoko ke umsebenzi wahluke kakhulu.Ngenxa yokuba sele iqulethe iprotheni yezilwanyana, kukho amathuba aphezulu eempawu ze-aleji.Indlela efunyenwe kwi-biochemically ye-thymus evela kwi-embryonic thymus entsha, enesivuno esiphantsi kakhulu kodwa isebenze kakuhle kakhulu.Ukuqwalasela izinto eziqingqiweyo ezikrwada, ixabiso eliphezulu, isivuno esiphantsi, kunye nenkqubo entsonkothileyo yokufunyanwa., ubunzima bemveliso enkulu, ithemba lophuhliso alinalo ithemba.
2. Ukwenziwa kweekhemikhali I-thymusfasin efumanekayo kurhwebo yenziwa ikakhulu njenge-SPSS, eveliswayo yaza yacocwa ngobuchule obufanelekileyo.Ngokusekwe kwi-resin, i-Fmoc yayisetyenziselwa ukubunjwa kwesigaba esiqinileyo, kunye neentlobo ze-28 ze-amino acids zenziwa ngokwe-thymalfasin ngokulandelelana.I-N-terminal amino acids yayi-acetylated kunye ne-acetic anhydride, kwaye i-TFA isetyenziswe njenge-reagent eqhekezayo ebalulekileyo yokuqhekeka ukufumana iimveliso ezinqabileyo.Imveliso ekrwada yahlulwe yi-HPLC ukufumana imveliso yendlela entsha ye-thymus, eyathi ke yahlanjululwa ngakumbi ukufumana imathiriyeli entsha ye-thymus ekrwada.
Zintathu iindidi zebhaktiriya eyenziwe ngokwemfuza
I-1) Iijeni ezininzi zixutywe kwinginginya ukuze zifumane iijene ezijoliswe kuzo, kwaye ekugqibeleni zibonakaliswe ngendlela yemizimba yokubandakanywa.Kuye kwaxelwa ukuba i-thymmethod inoveli ye-multi-string gene genes yasekwa nge-random annealing kunye nobuchule be-PCR kwaye iphindwe kwi-Escherichia coli.(2) I-protein ye-protein gene eyenziwe yahlanganiswa kwakhona kwimidiya, kwaye i-media yatshintshwa yaba ngumkhosi wokwakha iibhaktheriya ezenziwe ngofuzo.Ngokomzekelo, ngokusekwe kwi-peptide gene ye-signal kwi-plasmid, ibinzana elithi plasmid lakhiwa, laguqulwa laba yi-Pichia pastoris nge-electroexcitation, emva koko iprotein ye-fusion yenziwa yi-methanol, emva koko indlela entsha ye-thymus yakhiwa nge-enzyme digestion kunye nezinye iindlela. .
Ixesha lokuposa: Sep-08-2023