Ii-antigens zeprotheyini eziphinda zihlangane zihlala zineepitopes ezininzi ezahlukeneyo, ezinye zazo zii-epitopes ezilandelelanayo kwaye ezinye zii-epitopes zesakhiwo.Ii-antibodies ze-polyclonal ezifunyenwe ngokugonywa kwezilwanyana kunye ne-antigens e-denatured ziyimixube yee-antibodies ezikhethekileyo kwi-epitopes nganye kwaye inokusetyenziswa ngokubanzi ukufumanisa izakhiwo zendalo okanye iiprotheni ezijoliswe kuzo.Inzuzo yecala lokusebenzisa iiprotheyini ze-denatured njenge-immunogens kukuba iiprotheyini ze-denatured zivame ukuba zi-immunogenic kwaye zinokuvuselela impendulo enamandla yokuzivikela kwizilwanyana.
Inkqubo yokubonakalisa i-Escherichia coli idla ngokukhethwa kwiinjongo ze-antigenic kuba iyona nkqubo ibiza kakhulu ngokwexesha kunye nemali.Ukuze kuphuculwe ithuba lokubonakaliswa kweprotheyini ekujoliswe kuyo kunye nokulula ukuhlanjululwa, ngamanye amaxesha kuphela isiqwenga esincinci seprotheni ekujoliswe kuyo, njenge-domain ethile, ibonakaliswe.
Ulwakhiwo olunemigangatho emithathu yeeprotheni
Indawo eKhethekileyo yeProtheyini
Ukuba injongo yokulungiswa kwe-antibody kukufumana kuphela i-wb, kuqoqosho kwaye ngokukhawuleza ukusebenzisa i-peptide encinci ye-synthetic njenge-antigen, kodwa kukho umngcipheko we-immunogenicity ebuthathaka okanye i-non-regenecity ngenxa yokukhethwa okungafanelekanga kwecandelo le-peptide.Ekubeni ukulungiswa kwe-antibody kufuna ixesha elide, amacandelo amabini okanye amathathu ahlukeneyo e-peptide ahlala ekhethwa ukulungisa izilwa-buhlungu zisebenzisa i-polypeptide antigen ukuqinisekisa izinga lempumelelo yovavanyo.
Ukucoceka kwe-antigen ye-polypeptide yokugonywa kufuneka ibe ngaphezu kwe-80%.Nangona ucoceko oluphezulu lunokuthi lufumane ii-antibodies ezicacileyo ezingcono, ekusebenzeni, izilwanyana zihlala zivelisa inani elikhulu lee-antibodies ezingekho ngqo, ngaloo ndlela zifihla izibonelelo zokuhlanjululwa kwe-antigen.
Ukongeza, ukulungiswa kwee-antibodies ezivela kwi-peptides encinci kufuneka kudityaniswe ngokunqamlezileyo kwi-antigen efanelekileyo yokuthwala ukunyusa ukugonywa kwayo.Izithwali ezimbini ze-antigenic eziqhelekileyo yi-KLH kunye ne-BSA.
Ixesha lokuposa: Mar-23-2023