Ithini i-orientation yeepeptides ezenziwe ngokwezifiso?Ngaba uyazazi ezi ngongoma?

I-Peptide chain synthesis isetyenziswe ngokubanzi kwiinkalo ezininzi, ngakumbi kuphuhliso lwamachiza, uphando lwebhayoloji kunye ne-biotechnology.Iipeptide zobude obahlukeneyo kunye nolandelelwano zinokudityaniswa nge-peptide chain synthesis yokulungiswa kweziyobisi, umthwali weziyobisi, uhlalutyo lweprotheyini, uphando olusebenzayo njalo njalo.Ke lithini icala leepeptides zokwenziwa?Namhlanje, uGutuo Xiaobian uya kukunika impendulo ecacileyo ngezantsi.

Ithini i-orientation yeepeptides ezenziwe ngokwezifiso?Ngaba uyazazi ezi ngongoma?

I-Peptide chain synthesis inokufezekiswa ngokongezwa kwe-amino acid kwikhonkco elikhoyo le-peptide, ukuvelisa iibhondi ezintsha ze-peptide.Ngenxa yokukhula ukusuka ekupheleni kwe-N ukuya kwi-C peptide chain, ngoko i-direction of synthesis nayo isuka ekupheleni kwe-N ukuya kwi-C. Oku kungenxa yokuba i-C terminal kunye ne-N terminal ye-amino acids phakathi kwenkqubo yokwakheka kwe-peptide bond, i-amino acids ye-carboxyl end. (C) kunye ne-amino ekhoyo (N) ekupheleni kwe-peptide chain reaction, ibangela ibhondi entsha ye-peptide.Ke, isalathiso sendlela yokudibanisa ukusuka kwiN ukuya kwiC.

I-Peptide synthesis ikakhulu idibanisa iiamino acid molecule ngokusebenzisa indlela yekhemikhali ukwenza inkqubo yeepeptides.Ukuchaneka, i-polypeptide chain synthesis idibanisa iamino acid iimolekyuli, ngokulandelayo, inokusekwa ngamabhondi ekhemikhali ukwakha inkqubo yeepeptides.Ukwenziwa kwekhonkco lePeptide ngokuqhelekileyo kunokuqhutywa nokuba kukwinqanaba eliqinileyo okanye ulwelo lwenqanaba lokudibanisa.Kwi-slid-phase synthesis, ii-amino acids zokuqala zincanyathiselwe kwi-slid-phase material, kwaye ke ikhonkco le-peptide landiswa ngokulandelelana ngokongezwa kwe-amino acids.

Ngentla yintwana yokwenza ukwazisa ulwahlulo lwepeptide kunye nendima yolwazi olunxulumeneyo, i-tinto eyomeleleyo igxininise kwi-peptide synthesis, ukubonakaliswa kweprotheyini, ukulungiswa kwe-antibody, njl., ibandakanyeka kushishino lwe-peptide iminyaka emininzi, umgangatho wemveliso yodidi lokuqala. , wamkele inkitha yabathengi ukuba beze kubonana.