Yintoni indima yephosphorylation kwiipeptides?

I-Phosphorylation ichaphazela zonke iinkalo zobomi beselula, kunye neprotheyini kinases ichaphazela yonke imiba yonxibelelwano lwe-intracellular ngokulawula iindlela zokubonisa kunye neenkqubo zeselula.Nangona kunjalo, i-phosphorylation ephosakeleyo ikwangunobangela wezifo ezininzi;ngokukodwa, i-protein kinases eguquguqukayo kunye ne-phosphatases inokubangela izifo ezininzi, kwaye ezininzi i-toxins zendalo kunye ne-pathogens nazo zinefuthe ngokuguqula isimo se-phosphorylation yeeprotheni ze-intracellular.

I-Phosphorylation ye-serine (i-Ser), i-threonine (i-Thr), kunye ne-tyrosine (i-Tyr) yinkqubo yokuguqulwa kweprotheni eguqulwayo.Babandakanyeka ekulawuleni imisebenzi emininzi yeselula, njengokubonakaliswa kwe-receptor, i-protein association kunye ne-segmentation, i-activation okanye inhibition of protein function, kunye nokusinda kweseli.I-phosphates ihlawuliswa kakubi (iintlawulo ezimbini ezingalunganga kwiqela le-phosphate nganye).Ngoko ke, ukongezwa kwabo kuguqula iiprotheyini zeprotheyini, ngokuqhelekileyo inguqu ehambelanayo, ekhokelela ekutshintsheni kwisakhiwo seprotheni.Xa iqela le-phosphate lisusiwe, ukuhambelana kweprotheni kuya kubuyela kwimeko yayo yangaphambili.Ukuba iiprotheni ezimbini ezihambelanayo zibonisa imisebenzi eyahlukeneyo, iphosphorylation inokusebenza njengokutshintsha kwemolekyuli yeprotheni ukulawula umsebenzi wayo.

Amahomoni amaninzi alawula umsebenzi we-enzymes ethile ngokunyusa imeko ye-phosphorylation ye-serine (Ser) okanye i-threonine (Thr) i-residues, kunye ne-tyrosine (Tyr) i-phosphorylation inokubangelwa yizinto zokukhula (ezifana ne-insulin).Amaqela ephosphates ezi amino acids anokususwa ngokukhawuleza.Ke, iSer, iThr, kunye neTyr zisebenza njengotshintsho lwemolekyuli kulawulo lwemisebenzi yeselula efana nokunyuka kwethumba.

Iipeptide zokwenziwa zidlala indima eluncedo kakhulu kufundo lweprotein kinase substrates kunye nokusebenzisana.Nangona kunjalo, kukho izinto ezithile ezithintela okanye ezinciphisa ukuguquguquka kwetekhnoloji ye-phosphopeptide synthesis, njengokungakwazi ukuphumeza i-automation epheleleyo ye-slide-phase synthesis kunye nokungabikho koqhagamshelwano olufanelekileyo kunye namaqonga ohlalutyo aqhelekileyo.

I-platform esekelwe kwi-peptide synthesis kunye ne-phosphorylation modification technology inqoba le mida ngelixa iphucula i-synthesis ye-synthesis kunye ne-scalability, kwaye iqonga lifaneleke ngokufanelekileyo ukufundisisa i-protein kinase substrates, i-antigens, i-molecule ebophayo, kunye ne-inhibitors.